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KMID : 0370220090530030145
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Yakhak Hoeji
2009 Volume.53 No. 3 p.145 ~ p.150
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Proteomic Approach to the Cytotoxicity of 5-FU(Fluorouracil) in Colon Cancer Cells
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Lee Seo-Young
Song Jin-Soo
Roh Si-Hun
Kim Hee-Jun
Kwon Sung-Won
Park Jeong-Hill
Kim Geun-Tae
Hong Soon-Sun
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Abstract
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We evaluated cytotoxic effect based on the MTT assay and identified altered proteins in 5-FU(fluorouracil) treated HT29 cells using two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS. As proteins inducing apoptosis, siah binding protein 1 and p47 protein isoform a were up-regulated and tumor protein translationally-controlled 1 was down-regulated by 5-FU treatment. And mannose 6 phosphate receptor binding protein 1 controls DNA mismatch repair system was increased. We suggest 5-FU promotes a cytotoxicity under the action of these proteins in colon cancer cells.
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KEYWORD
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5-FU, colon cancer, proteomics, MALDI-TOF/TOF
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